2010 to Present

Tocilj, K. F. On, Z. Yuan, J. Sun, E. Elkayam, H. Li, B. Stillman* and L. Joshua-Tor*, Structure of the active form of human Origin Recognition Complex and its ATPase motor module, eLife (2017);6:e20818. doi: 10.7554/eLife.20818.

J. J. Ipsaro, C. Shen C, E. Arai, Y. Xu, J. B. Kinney, L. Joshua-Tor, C. R. Vakoc and J. Shi, Rapid generation of drug resistance alleles at endogenous loci using CRISPR-Cas9 indel mutagenesis, PLoS ONE 12(2) (2017):e0172177. doi:10.1371/journal.pone.0172177

E. Elkayam, R. Parmar, C. R. Brown, J. L. Willoughby, C. S. Theile, M. Manoharan*, L. Joshua-Tor*, siRNA carrying an (E)-vinylphosphonate moiety at the 5’ end of the guide strand augments gene silencing by enhanced binding to human Argonaute-2, Nucl. Acids Res. (2016). doi: 10.1093/nar/gkw1171.

C. Shen, J. J. Ipsaro, J. Shi, J. A. Milazzo, E. Wang, J.-S. Roe, Y. Suzuki, D. J. Pappin, L. Joshua-Tor, and C. R. Vakoc, NSD3-short is an adaptor protein that couples BRD4 to the CHD8 chromatin remodeler, Mol. Cell, 60, published online November 25, 2015.

Naguib, Adam and Bencze, Gyula and Cho, Hyejin and Zheng, Wu and Tocilj, Ante and Elkayam, Elad and Faehnle, Christopher R and Jaber, Nadia and Pratt, Christopher P and Chen, Muhan and Zong, Wei-Xing and Marks, Michael S and Joshua-Tor, Leemor and Pappin, Darryl J and Trotman, Lloyd C (2015) PTEN Functions by Recruitment to Cytoplasmic Vesicles. Molecular Cell, 58 (2). pp. 255-268. ISSN 1097-2765

Kuhn, C. D. and Wilusz, J. E. and Zheng, Y. and Beal, P. A. and Joshua-Tor, L. (2015) On-Enzyme Refolding Permits Small RNA and tRNA Surveillance by the CCA-Adding Enzyme. Cell, 160 (4). pp. 644-658. ISSN 0092-8674 4X4N, 4X4O, 4X4P, 4X4Q, 4X4R, 4X4S, 4X4T, 4X4U, 4X4V, 4X4W

Ipsaro, J. J. and Joshua-Tor, L. (2015) From guide to target: molecular insights into eukaryotic RNA-interference machinery. Nat Struct Mol Biol, 22 (1). pp. 20-28. ISSN 1545-9985. Invited review.

Faehnle, C. R. and Walleshauser, J. and Joshua-Tor, L. (2014) Mechanism of Dis3l2 substrate recognition in the Lin28-let-7 pathway. Nature, 514 (7521). pp. 252-6. ISSN 0028-0836 4PMW

Lee, S. J. and Syed, S. and Enemark, E. J. and Schuck, S. and Stenlund, A. and Ha, T. and Joshua-Tor, L. (2014) Dynamic look at DNA unwinding by a replicative helicase. Proceedings of the National Academy of Sciences of the United States of America, 111 (9). E827-35. ISSN 0027-8424.

Faehnle, C. R. and Elkayam, E. and Haase, A. D. and Hannon, G. J. and Joshua-Tor, L. (2014) Slicer and the Argonautes. Biophysical Journal, 106 (2; Sup). 455A-455A. ISSN 0006-3495. Meeting abstract.

Kuscu, C. and Zaratiegui, M. and Kim, H. S. and Wah, D. A. and Martienssen, R. A. and Schalch, T. and Joshua-Tor, L. (2014) CRL4-like Clr4 complex in Schizosaccharomyces pombe depends on an exposed surface of Dos1 for heterochromatin silencing. Proc Natl Acad Sci U S A. ISSN 0027-8424 4O9D

Faehnle, Christopher R and Elkayam, Elad and Haase, Astrid D and Hannon, Gregory J and Joshua-Tor, Leemor (2013) The Making of a Slicer: Activation of Human Argonaute-1. Cell Reports, 3 (6). pp. 1901-1909. ISSN 2211-1247 4KRE, 4KRF

Naguib, A. and Bencze, G. and Faehnle, C. R. and Schalch, T. and Lazar, Z. and Ruse, C. I. and Joshua-Tor, L. and Pappin, D. J. and Trotman, L. C. (2013) Upstream activation of PTEN. Cancer Research, 73 (8 (Sup). p. 5156. ISSN 0008-5472. Meeting abstract.

Kuhn, C. D. and Joshua-Tor, L. (2013) Eukaryotic Argonautes come into focus. Trends in Biochemical Sciences, 38 (5). pp. 263-271. ISSN 09680004 (ISSN)

Paul, S. and Kuo, A. and Schalch, T. and Vogel, H. and Joshua-Tor, L. and McCombie, W. R. and Gozani, O. and Hammell, M. and Mills, A. A. (2013) Chd5 Requires PHD-Mediated Histone 3 Binding for Tumor Suppression. Cell Reports, 3 (1). pp. 92-102. ISSN 2211-1247

Wilusz, J. E. and Jnbaptiste, C. K. and Lu, L. Y. and Kuhn, C. D. and Joshua-Tor, L. and Sharp, P. A. (2012) A triple helix stabilizes the 3′ ends of long noncoding RNAs that lack poly(A) tails. Genes Dev, 26 (21). pp. 2392-407. ISSN 1549-5477 (Electronic)0890-9369 (Linking)

Lavy, T. and Kumar, P. R. and He, H. Z. and Joshua-Tor, L. (2012) The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation. Genes & Development, 26 (3). pp. 294-303. ISSN 0890-9369 3V2U, 3V5R view pdf

Ipsaro, J. J. and Haase, A. D. and Knott, S. R. and Joshua-Tor, L. and Hannon, G. J. (2012) The structural biochemistry of Zucchini implicates it as a nuclease in piRNA biogenesis. Nature, 491 (7423). pp. 279-282. ISSN 00280836 (ISSN) 4GGJ, 4GGK

Elkayam, E. and Kuhn, C. D. and Tocilj, A. and Haase, A. D. and Greene, E. M. and Hannon, G. J. and Joshua-Tor, L. (2012) The structure of human argonaute-2 in complex with miR-20a. Cell, 150 (1). pp. 100-110. ISSN 00928674 (ISSN) 4F3T, view pdf

Joshua-Tor, L. and Hannon, G. J. (2011) Ancestral Roles of Small RNAs: An Ago-Centric Perspective. Cold Spring Harb Perspect Biol. ISSN 1943-0264 (Electronic)

Schalch, T. and Job, G. and Shanker, S. and Partridge, J. F. and Joshua-Tor, L. (2011) The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS. Nature Structural and Molecular Biology, 18 (12). pp. 1351-1357. ISSN 15459993 (ISSN) 3TIX

Faehnle, C. R. and Joshua-Tor, L. (2010) Argonaute MID domain takes centre stage. EMBO Reports, 11 (8). pp. 564-5. ISSN 1469-221X. Invited abstract.


T. Schalch, G. Job, V. J. Noffsinger, S. Shanker, C. Kuscu, L. Joshua-Tor*, and J. F. Partridge*, High-affinity binding of Chp1 chromodomain to K9 methylated histone H3 is required to establish centromeric heterochromatin, Mol. Cell, 34, 36-46 (2009). 3G7L view pdf

P. R. Kumar, Y. Yu, R. Sternglanz, S. A. Johnston and L. Joshua-Tor, NADP regulates the yeast GAL induction system, Science, 319, 1090-1092 (2008). 3BTS, 3BTV, 3BTU. view pdf

E. J. Enemark and L. Joshua-Tor, On helicases and other motor proteins, Curr. Opin. Struc. Biol., 18, 243-257 (2008). doi: 10.1016/j.sbi.2008.01.007. Invited review article.

Christopher R. Faehnle and Leemor Joshua-Tor, Argonaute confronts new small RNAs, Curr. Opin. Chem. Biol., 11, 569-577 (2007). doi: 10.1016/jicbpa.2007.08.032. Invited review article.

N.H. Tolia and L. Joshua-Tor, Slicer and the Argonautes, Nature Chem. Biol., 3(1), 36-43 (2007). doi: 10.1038/nchembio848.

E. Yigit, P. J. Batista, Y. Bei, K. M. Pang, C. G. Chen, N. H. Tolia, L. Joshua-Tor, S. Mitani, M. J. Simard and C. C. Mello, Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi, Cell, 127(4), 747-57 (2006). doi: 10.1016/j.cell.2006.09.033

P. A. O’Farrell and L. Joshua-Tor, Mutagenesis and crystallographic studies of the catalytic residues of the papain family protease Bleomycin Hydrolase: new insights into active-site structure, Biochem. J., 401, 421-428 (2007). doi: 10.1042/BJ20060641; published online September 28, 2006.

E. J. Enemark and L. Joshua-Tor, Mechanism of DNA translocation in a replicative hexameric helicase, Nature, 442, 270-275 (2006). DOI:10.1038/nature04943.2GXA

D. V. Irvine*, M. Zaratiegui*, N. H. Tolia*, D. B. Goto, D. H. Chitwood, M. W. Vaughn, L. Joshua-Tor and R. A. Martienssen, Argonaute slicing is required for heterochromatic silencing and spreading, Science, 313, 1134-1137 (2006). doi: 10.1126/science.1128813.

Leemor Joshua-Tor, The Argonautes, Cold Spring Harb Symp Quant Biol., 71, 67-72 (2006). Invited review article.

J. J. Song and L. Joshua-Tor, Argonaute and RNA – getting into the groove, Curr. Opin. Struc. Biol., 16, 5-11 (2006). doi: 10.1016/j.sbi.2006.01.010.view pdf.

N. H. Tolia and L. Joshua-Tor, Strategies for protein coexpression in Escherichia coli, Nature Methods, 3(1), 55-64 (2006). doi: 10.1038/nmeth0106-55.view pdf.

N. H. Tolia, E. J. Enemark, B. K. L. Sim and L. Joshua-Tor, Structural basis for the EBA-175 erythrocyte invasion pathway of the malaria parasite Plasmodium falciparum, Cell, 122, 183-193 (2005) (10.1016/j.cell.2005.05.033). 1ZRL, 1ZRO, view pdf. supplementary information

F. V. Rivas, N. H. Tolia, J. J. Song, J. P. Aragon, J. Liu, G. J. Hannon and L. Joshua-Tor, Purified Argonaute2 and an siRNA form recombinant human RISC,
Nature Struc. Mol. Biol., 12(4), 340-349 (2005). published online 30 march, 2005 (10.1038/nsmb918). 1Z25, 1Z26, view pdf. supplementary information

J. J. Song, S. K. Smith, G. J. Hannon and L. Joshua-Tor, Crystal structure of Argonaute and its implications for RISC Slicer activity, Science, 305, 1434-1437 (2004); published online July 29, 2004 (10.1126/science.1102514). 1U04 , view pdf. supplementary information J. Liu, M. A. Carmell, F. V. Rivas, C. G. Marsden, J. M. Thomson, J. J. Song, S. M. Hammond, L. Joshua-Tor and G. J. Hannon, Argonaute2 is the catalytic engine of mammalian RNAi, Science, 305, 1437-1441 (2004); published online July 29, 2004 (10.1126/science.1102513). view pdf. supplementary information

L. Joshua-Tor, siRNAs at RISC, Structure, 12 (7), 1120-1122 (2004). view pdf

Leemor Joshua-Tor and Stephen A. Johnston, The Bleomycin Hydrolases, in Handbook of Proteolytic Enzymes 2nd edition (A.J. Barrett, N.D. Rawlings and J. F. Woessner eds.), pp. 1197-1201, Elsevier, London (2004). view pdf

A. S. Auster and L. Joshua-Tor, The DNA-binding domain of HPV-18 E1: crystal structure, dimerization and DNA binding. J. Biol. Chem., 279 (5), 3733-3742 (2004). E-pub: October 30, 2003. 1R9W , view pdf

J. J. Song, J. Liu, N. H. Tolia, J. Schneiderman, S. K. Smith, R. A. Martienssen, G. J. Hannon and L. Joshua-Tor, The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes. Nat. Struct. Biol., 10 (12), 1026-1032 (2003). 1R6Z , view pdf. supplementary information

T. E. Messick, N. H. Chmiel, M. Golinelli, M. R. Langer, L. Joshua-Tor and S. S. David, Noncysteinyl coordination to the [4Fe-4S]2+ Cluster of the DNA Repair Adenine Glycosylase MutY introduced via Site-Directed Mutagenesis.  Structural Characterization of an Unusual Histidinyl Coordinated Cluster. Biochemistry, 41(12), 3931-3942 (2002). 1KQJ , view pdf

E. J. Enemark, A. Stenlund and L. Joshua-Tor, Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex, EMBO J., 21(6), 1487-1496 (2002). 1KSY, 1KSX , view pdf

D. M. F. van Aalten, W. Crielaard, K. J. Hellingwerf and L. Joshua-Tor, Structure of the photoactive yellow protein reconstituted with caffeic acid at 1.16 Å resolution, Acta Crystallogr. D., 58(4), 585-590 (2002). 1KOU , view pdf

D. M. F. van Aalten, A. Haker, J. Hendriks, K. J. Hellingwerf, L. Joshua-Tor and W. Crielaard, Engineering photocycle dynamics: crystal structures and kinetics of threephotoactive yellow protein hinge bending mutants, J. Biol. Chem., 277(8), 6463-6468 (2002). 1GSV ,1GSW ,1GSX , view pdf

S. J. Russell, F. Gonzalez, L. Joshua-Tor and S. A. Johnston, Selective Chemical Inactivation of AAA Proteins Reveals Distinct Functions of Proteasomal ATPases, Chem. & Biol. , 8, 941-950 (2001). view pdf

E. J. Enemark, G. Y. Chen, D. E. Vaughn, A. Stenlund and L. Joshua-Tor , Crystal Structure of the DNA-Binding Domain of the Replication Initiation Protein E1 from Papillomavirus, Mol. Cell , 6, 146-158(2000). 1F08 , view pdf

D. M. F. van Aalten, C. C. Chong and L. Joshua-Tor , Crystal Structure of Carboxypeptidase A Complexed with D-Cysteine at 1.75 A – Inhibitor-Induced Conformational Changes, Biochemistry , 39(33), 10082-10089(2000). 1F57 , view pdf

D. M. F. van Aalten, W. Crielaard, K. J. Hellingwerf, and L. Joshua-Tor , Conformational Substates in Different Crystal Forms of the Photoactive Yellow Protein – Correlation with Theoretical and Experimental Flexibility, Protein Sci ,9, (1), 64-72 (2000). 1D7E , view pdf

1999 and earlier

D. M. F. van Aalten, D. A. Erlanson, G. L. Verdine and L. Joshua-Tor , A Structural Snapshot of Base Pair Opening in DNA, Proc. Natl. Acad. Sci., USA ,96 , 11809-11814 (1999). 1CW9 , view pdf

D. E. Vaughn, J. Rodriguez, Y. Lazebnik and L. Joshua-Tor , Crystal Structure of the Apaf-1 Caspase Recruitment Domain: An a-Helical Greek Key Fold for Apoptotic Signaling, J. Mol. Biol ., 293 , 439-447 (1999). 1CY5 , view pdf

P. A. O’Farrell, F. Gonzalez, W. Zheng, S. A. Johnston and L. Joshua-Tor , Crystal Structure of Human Bleomycin Hydrolase, a Self-Compartmentalizing Cysteine Protease, Structure ,7(6), 619-627 (1999). 1CB5 , 2CB5 , view pdf

W. Zheng, S. A. Johnston and L. Joshua-Tor , The Unusual Active Site of Gal6/Bleomycin Hydrolase Can Act as a Carboxypeptidase, Aminopeptidase, and Peptide Ligase, Cell ,93 , 103-109 (1998). 1A6R , 3GCB , view pdf

D. M. F. van Aalten, E. Grotewold and L. Joshua-Tor , Essential Dynamics from NMR Clusters: Dynamic Properties of the Myb DNA-Binding Domain and a Hinge- Bending Enhancing Variant, Methods , 14(3), 318-328 (1998). view pdf

L. Joshua-Tor and S. A. Johnston, The Bleomycin Hydrolases, in Handbook of Proteolytic Enzymes (A.J. Barrett, N.D. Rawlings and J. F. Woessner eds.), pp. 634-638, Academic Press , London (1998).

J. L. Schlessman, D. Woo, L. Joshua-Tor , J. B. Howard and Douglas C. Rees, Conformational Variability in Structures of the Nitrogenase Iron Proteins from Azotobacter vinelandii and Clostridium pasteurianum , J. Mol. Biol ., 280 , 669-685 (1998). 1CP2 , 2NIP , view pdf

L. Joshua-Tor , H.E. Xu, S.A. Johnston and D.C. Rees, Crystal Structure of a Conserved Protease That Binds DNA: The Bleomycin Hydrolase, Gal6. Science 269 , 945-950 (1995). 1GCB

L. Joshua-Tor and J.L. Sussman, The coming of Age of DNA Cyrstallography, Current Opinions in Structural Biology 3, 323-335 (1993).

D.C. Rees, J. Kim, M.M. Geogiadis, H. Komiya, A.J. Chirino, D. Woo, J. Schlessman. M.K. Chan , L. Joshua-Tor , G. Santillan, P. Chakrabarti and B.Y. Hsu. Crystal Structures of the Iron Protein and Molybdenum-Iron Protein of Nitrogenase, in ACS Symposium on Molybdenum Enzymes ,Cofactors and Model Systems (E.I. Stiefl, D. Coucouvanis and W.E. Newton, eds.), Series 535, 170-175 (1993).

P.R. Blake, M.W. Day, B.T. Hsu, L. Joshua-Tor , J.-B. Park, D.R. Hare, M.W.W. Adams, D.C. Rees and M.F. Summers, Comparison of the X-ray Structure of Native Rubredoxin from P. furiosus with the NMR Structure of the Zinc-Substituted Protein, Protein Science , 1, 1522-1525 (1992). view pdf

M.W. Day, B.T. Hsu, L. Joshua-Tor , J.-B. Park, Z.H. Zhou, M.W.W. Adams and D.C. Rees, X-ray Crystal Structures of the Oxidized and Reduced Forms of the Rubredoxin from the Marine Hyperthermolphilic Archaebacterium Pyrococcus furiosus, Protein Science 1, 1494-1507 (1992). 1CAA , 1CAD , view pdf

H.M. Berman, J.L. Sussman, L. Joshua-Tor , C.G. Revich and L.S. Ripley, A Structural Model for Sequence-Specific Proflavin-DNA Interactions During In Vitro Frameshift Mutagenesis, J. Biomol. Struc. Dyn. 10, 317-331 (1992).

L. Joshua-Tor , F. Frolow, E. Appella, H. Hope, D. Rabinovich and J.L.Sussman, The Three-Dimensional Structures of Bulge-Containing DNA Fragments, J. Mol. Biol. 225, 397-431 (1992). 1D31

J.L. Sussman, L. Joshua-Tor , M. Hirshberg, M.A. Saper, F. Frowlow, H. Hope and E. Appella, A Kinked Model of a DNA Tridecamer with an Unpaired Adenosine: Energy Minimization and X-ray Structural Studies, in Molecular Structure: Chemical Reactivity and Biological Activity (Stezowski, J.J., ed.), 195-200, Oxford University Press (1988).

L. Joshua-Tor , D. Rabinovich, H. Hope, F. Frolow, E. Appella and J.L. Sussman, The Three-Dimensional Structure of a DNA Duplex Containing Looped Out Bases, Nature 334, 82-85 (1988). 1D31.